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Polyacrolein microspheres were prepared by radiation polymerization of acrolein in the absence of emulsifying or stabilizing agent. The microspheres had functional surface aldehyde groups permitting covalent binding with antibody in 1 step. The particle size of the microspheres varied with polymerization conditions, especially irradiation temperature. The microsphere antibody conjugates obtained by binding immunoglobulins to polyacrolein microspheres were used to label cells. The reactivity of microsphere antibody conjugates was shown by specific aggregation by antigen.
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