Linked Life Data

6352812

Source:http://linkedlifedata.com/resource/pubmed/id/6352812

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-11-23
pubmed:abstractText
An immunoenzyme procedure is described for detection of proteins separated by SDS-polyacrylamide gel electrophoresis. Proteins were electrophoretically transferred to nitrocellulose filters which were then sequentially incubated with mouse monoclonal antibody, alkaline-phosphatase-conjugated antibody to mouse immunoglobulins and a substrate mixture containing beta-naphthyl phosphate and Fast Blue B. The procedure has been successfully applied to several monoclonal antibodies of various heavy chain classes and can detect protein antigens at concentrations down to 0.5 ng/mm2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
The use of alkaline-phosphatase-conjugated second antibody for the visualization of electrophoretically separated proteins recognized by monoclonal antibodies.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't