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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1983-10-28
|
| pubmed:abstractText |
Genetical analysis of two-spored asci formed by interrupted sporulation offers a novel procedure for mapping of centromere-linked genes in Saccharomyces cerevisiae. Unlike the two-spored asci encountered under normal sporulation conditions, these asci are produced by a nonrandom mechanism. They fall into three categories (+ +), (+ -) and (- -) with respect to any marker. The percentage of (+ -) asci varies directly as a function of centromere-linkage of a gene. It is observed that almost 100% asci are of the (+ -) type in case of very tightly linked genes like trp-1 and cdc-10, while in case of markers unlinked to the centromere, e.g, trp-5 and met-8, the (+ -) asci constitute 50% of the total number of asci. Other markers with varying degrees of linkage, e.g. ura-3 and lys-1 show corresponding numbers of (+ -) asci between 50% and 100% of the total asci. These findings are in contrast to the results expected from a random abortion of two spores, in which case the (+ -) asci would constitute 67% of the total number of asci irrespective of the degree of centromere linkage of a marker. The linkage-dependent segregation of markers in these new kind of two-spored asci permits a rapid and accurate estimate of centromere linkage of a gene.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0026-8925
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
191
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
165-6
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading | |
| pubmed:year |
1983
|
| pubmed:articleTitle |
Two-spored asci produced by interrupted sporulation: a novel approach to linkage analysis in yeast.
|
| pubmed:publicationType |
Journal Article
|