Linked Life Data

6348038

Source:http://linkedlifedata.com/resource/pubmed/id/6348038

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1983-9-9
pubmed:abstractText
We have measured the movement of newly synthesized phosphatidylethanolamine (PE) molecules from sites of intracellular synthesis to the plasma membrane in cultured V79 Chinese hamster fibroblasts. Plasma membrane PE was distinguished from intracellular PE by its derivatization with an amino-reactive reagent, trinitrobenzene sulfonic acid, under nonpermeating conditions. Within minutes after the addition of radiolabeled precursors of PE to the culture medium, radiolabeled PE appeared at the plasma membrane. The fraction of radiolabeled PE molecules appearing at the plasma membrane increased rapidly over a 2-h period and then increased very slowly for several days to a constant specific radioactivity. By measuring the release of radiolabeled secretory proteins, we determined that the transport of newly synthesized proteins to the cell surface occurred more slowly than the transport of PE. Preincubation of cells with either cytochalasin B, cytochalasin D, colchicine, oncobendazole, sodium azide, 2-deoxyglucose, dinitrophenol, p-trifluoromethoxyphenylhydrazone, or monensin did not block the transport of de novo synthesized PE; however, incubation of cells in culture medium at 2 degrees C effectively halted the appearance of new PE molecules at the plasma membrane. When cells which had been incubated at 2 degrees C were warmed, PE molecules from intracellular PE pools once again began to appear at the plasma membrane. These results suggest that the rapid transport of newly synthesized PE molecules to the plasma membrane occurs by a mechanism independent of that used for the transport of newly synthesized proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9050-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Rapid appearance of newly synthesized phosphatidylethanolamine at the plasma membrane.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't