Linked Life Data

6347912

Source:http://linkedlifedata.com/resource/pubmed/id/6347912

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-9-20
pubmed:abstractText
The presence of fibronectin in three "malignant" (AU-471, AU-436, LT-2) and two "benign" (BHK-21, WI-38) cell lines was demonstrated with a fluorescent antibody technique; two malignant (AU-471, AU-436) cell lines were fibronectin-negative and one (LT-2) retained fibronectin expression. One "benign" cell line (WI-38) expressed fibronectin, the other (BHK-21) did not. Anchorage-independent soft agar (AISA) growth correlated better with loss of fibronectin than with malignant potential. All three fibronectin-negative cell lines (benign and malignant) grew anchorage-independently (AU-471, AU-436, BHK-21), and both fibronectin-positive cell lines were anchorage-dependent (LT-2, WI-38). Surprisingly, the addition of Clg to anchorage-independent cells increased their anchorage-independent soft-agar cloning efficiency, but had no effect on anchorage-dependent cell lines. Anti-Clg antibodies decreased AISA growth. The effect of Clg on anchorage-independent growth varied with the concentration, and also between cell lines, and a variation in effect was noted between anchorage-independent (AISA) and anchorage-dependent (in flasks) growth even in the same cell line.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Fibronectin and anchorage-independent and anchorage-dependent growth of benign and malignant cell lines.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.