Linked Life Data

6346578

Source:http://linkedlifedata.com/resource/pubmed/id/6346578

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-8-11
pubmed:abstractText
Highly purified human prothrombin was activated by Ecarin, a prothrombin activating principle of Echis carinatus venom and the generated thrombin-like activity was investigated. Kinetics of the release of fibrino-peptides A and B (FPA, FPB) from human fibrinogen was estimated using radioimmunoassay technique. The direct proteolytic action of Ecarin on fibrinogen was studied by means of polyacrylamide gel electrophoresis and by radioimmunoassay for fragment B beta 15-42 of fibrinogen. In a system containing Ecarin, fibrinogen and prothrombin both fibrinopeptides were cleaved off at a rate that was essentially similar to that observed with thrombin, the cleavage off of FPB being in the initial stage always slower than the release of FPA. At physiological concentration of prothrombin and at low concentration of Ecarin all FPB were liberated while less than 1% of B beta 15-42 immunoreactivity was released. It was possible to accelerate the release of FPB when prothrombin was activated by Ecarin in the presence of Fibrin I gel (formed from fibrinogen by Batroxobin which liberates FPA only) instead of fibrinogen. Fibrin I thus constituted a more favourable substrate than fibrinogen with regard to release of FPB. The results indicate that the coagulant activity generated from prothrombin by Ecarin is thrombin-like. No intermediate product capable of marked cleavage off of FPA only was detected.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0049-3848
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
225-34
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
The action of prothrombin activated by Ecarin on fibrinogen.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't