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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1983-7-29
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pubmed:abstractText |
The hemolytic Escherichia coli strain 536 (O6) propagates spontaneous hemolysin-negative mutants at relatively high rates (10(-3) to 10(-4)). One type of mutant (type I) lacks both secreted (external) and periplasmic (internal) hemolysin activity (Hlyex-/Hlyin-) and in addition shows no mannose-resistant hemagglutination (Mrh-), whereas the other type (type II) is Hlyex-/Hlyin+ and Mrh+. The genetic determinants for hemolysin production (hly) and for mannose-resistant hemagglutination (mrh) of this strain are located on the chromosome. Hybridization experiments with DNA probes specific for various parts of the hly determinant reveal that mutants of type I have lost the total hly determinant, whereas those of type II lack only part of the hlyB that is essential for transport of hemolysin across the outer membrane. Using a probe that contains the end sequence of the plasmid pHly152-encoded hly determinant (adjacent to hlyB), we determined that a related sequence flanks also the hlyB-distal end of the chromosomal hly determinant of E. coli 536. In addition several other similar or even identical sequences are found in the vicinity of the hlyC- and the hlyB-distal ends of both the chromosomal and the plasmid hly determinants.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-105244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-22493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-352937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-368646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-392234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-4559594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-4605400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-4929855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-5340526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-6257634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-6257652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-6259123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-6279020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-6282809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-6754701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-6998968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-7007421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-7011993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-7012012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-7014607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-7021838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-7031483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-7050085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-797531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6343344-881736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
154
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1145-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6343344-Base Sequence,
pubmed-meshheading:6343344-Chromosomes, Bacterial,
pubmed-meshheading:6343344-Escherichia coli,
pubmed-meshheading:6343344-Genes, Bacterial,
pubmed-meshheading:6343344-Hemagglutination,
pubmed-meshheading:6343344-Hemolysin Factors,
pubmed-meshheading:6343344-Hemolysin Proteins,
pubmed-meshheading:6343344-Mannose,
pubmed-meshheading:6343344-Nucleic Acid Hybridization
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pubmed:year |
1983
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pubmed:articleTitle |
Spontaneous deletions and flanking regions of the chromosomally inherited hemolysin determinant of an Escherichia coli O6 strain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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