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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1983-5-27
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pubmed:abstractText |
A monoclonal antibody, PHM 5, directed against human glomerular epithelial cells, was prepared after immunisation of a mouse with isolated human glomeruli and fusion of spleen cells with a mouse myeloma. Binding of antibody to isolated glomeruli was detected by radioimmune indirect binding assay, and specificity for glomerular epithelial cells was shown using a four-layer peroxidase-antiperoxidase technique applied to epoxy resin sections of the human kidney cortex. PHM 5 appears to detect a human-specific cell surface carbohydrate antigen not previously described, and can be used to identify epithelial cells in renal biopsy sections and in culture outgrowths of isolated glomeruli.
|
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:issn |
0028-2766
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
33
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
83-90
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:6339971-Animals,
pubmed-meshheading:6339971-Antibodies, Monoclonal,
pubmed-meshheading:6339971-Antibody Specificity,
pubmed-meshheading:6339971-Antigens, Surface,
pubmed-meshheading:6339971-Binding Sites, Antibody,
pubmed-meshheading:6339971-Epithelium,
pubmed-meshheading:6339971-Humans,
pubmed-meshheading:6339971-Hybridomas,
pubmed-meshheading:6339971-Immunoenzyme Techniques,
pubmed-meshheading:6339971-Kidney Glomerulus,
pubmed-meshheading:6339971-Mice
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pubmed:year |
1983
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pubmed:articleTitle |
Monoclonal antibodies to human glomerular cells: a marker for glomerular epithelial cells.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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