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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1983-4-15
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pubmed:databankReference |
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pubmed:abstractText |
The gene for protein A from Staphylococcus aureus was cloned into pBR322 in Escherichia coli. An immunoassay was used to detect production of the protein. Protein A produced in E. coli was found in the periplasmic space and was purified and concentrated by IgG-Sepharose affinity chromatography. DNA sequence assay of the gene revealed a region with the general features of a prokaryotic signal peptide and a fifth structural region homologous to the four repetitive regions found earlier by amino acid sequence determination of the mature protein. Upstream from the structural gene there is a possible promoter region and a ribosomal binding sequence typical of gram-positive bacteria. The initiation codon is TTG.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-1090574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-125746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-13906598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-344137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-349081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-352503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-396437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4163007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4264698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4284300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4304946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4448166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-448802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4517042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4589344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4630462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4767461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-4896022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-5083510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-5171042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-585340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-6170539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-6248430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-6260582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-6260584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-6793593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-6977472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-6993100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-7006445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-7037399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-803646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-94251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6338496-964266
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
697-701
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1983
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pubmed:articleTitle |
Gene for staphylococcal protein A.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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