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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1984-10-25
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pubmed:abstractText |
Two cloned repetitive DNA probes, pXBR and CY1, which bind preferentially to specific regions of the human X and Y chromosome, respectively, were used to study the distribution of the sex chromosomes in human lymphocyte nuclei by in situ hybridization experiments. Our data indicate a large variability of the distances between the sex chromosomes in male and female interphase nuclei. However, the mean distance observed between the X and Y chromosome was significantly smaller than the mean distance observed between the two X-chromosomes. The distribution of distances determined experimentally is compared with three model distributions of distances, and the question of a non-random distribution of sex chromosomes is discussed. Mathematical details of these model distributions are provided in an Appendix to this paper. In the case of a human translocation chromosome (Xqter----Xp22.2::Yq11----Yqter) contained in the Chinese hamster X human hybrid cell line 445 X 393, the binding sites of pXBR and CY1 were found close to each other in most interphase nuclei. These data demonstrate the potential use of chromosome-specific repetitive DNA probes to study the problem of interphase chromosome topography.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0340-6717
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
317-25
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:6336321-Cell Nucleus,
pubmed-meshheading:6336321-Centromere,
pubmed-meshheading:6336321-Female,
pubmed-meshheading:6336321-Humans,
pubmed-meshheading:6336321-Interphase,
pubmed-meshheading:6336321-Lymphocytes,
pubmed-meshheading:6336321-Male,
pubmed-meshheading:6336321-Nucleic Acid Hybridization,
pubmed-meshheading:6336321-Sex Chromosomes
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pubmed:year |
1984
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pubmed:articleTitle |
Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes.
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pubmed:publicationType |
Journal Article
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