Linked Life Data

63362

Source:http://linkedlifedata.com/resource/pubmed/id/63362

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1977-2-26
pubmed:abstractText
Dotted chromosomes were consistently produced in both BrdU and non-BrdU substituted Chinese hamster cells after treatment with 1.0 M Na-phosphate solution, adjusted to pH 9.0 with a supersaturating amount of NaHCO3, and at a temperature of 80--95 degrees C. -- A series of changes in chromosome morphology was produced as the temperature of the solution was progressively increased. In BrdU-treated cells, G-banding and differentially stained sister chromatids were sequentially produced prior to the appearance of dots. In non-BrdU treated cells, only G-banding was produced before dot formation. In general, the patterns of dots correspond to the G-banding patterns. --Chromatids, with uni- or bifilarly BrdU substituted DNA or with normal DNA, required differential temperatures for the production of dots. Since the temperature required for dot formation was always slightly higher than that required for producing differentially stained chromatids, this phenomenon can be used as an important indicator for determining the optimal temperature required for revealing differentially stained chromatids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0009-5915
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-7
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Dotted chromosomes produced in sodium phosphate solution supersaturated with NaHCO3.
pubmed:publicationType
Journal Article