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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0000431,
umls-concept:C0003250,
umls-concept:C0010762,
umls-concept:C0017262,
umls-concept:C0031412,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0205369,
umls-concept:C0677626,
umls-concept:C0936012,
umls-concept:C1882726,
umls-concept:C2911684
|
| pubmed:issue |
3
|
| pubmed:dateCreated |
1985-2-8
|
| pubmed:abstractText |
We have studied the expression of aldrin eposidase (AE), 7-ethoxycoumarin-O-deethylase (ECDE), and aryl hydrocarbon (benzo[a]pyrene) hydroxylase (AHH) in nine differentiated or dedifferentiated cell lines derived from H4IIEC3 rat hepatoma cells. The nature of the cytochromes P-450 mediating AE, ECDE and AHH activities was analysed using monoclonal antibodies (MAb) made to the major 3-methylcholanthrene-induced cytochrome P-450 (MAb-MC) or phenobarbital-induced cytochrome P-450 (MAb-PB) from rat liver. The cells were treated with 5 microM dexamethasone for 30 h to increase the levels of the monoxygenase activities. (a) The six differentiated cell lines examined (Faza967, Fao, HF1-4, 2sFou, C2Rev7, and H4IIEC3/G-) contained MAb-PB-sensitive AE comprising 30-75% of the total AE activity. In most of these cell lines MAb-PB also markedly inhibited ECDE; however, the antibody had a considerably weaker effect on AHH. (b) MAb-PB-sensitive AHH, ECDE and AE activities were also observed in untreated and phenobarbital-treated cells. (c) MAb-MC inhibited AHH and ECDE in the two dedifferentiated lines HF1 and H5 by 50-80%. The antibody also inhibited AHH activities in the poorly differentiated line H4IIEC3/T and in the majority of the differentiated lines by 40-65%. MAb-MC-sensitive AHH was found in Fao cells after treatment with benz[a]anthracene but induced AHH in H4IIEC3/T, H4IIEC3/G-, and 2sFou cells 20-30-fold and in Faza967 and Fao cells 3-5-fold. Benz[a]anthracene remained without effect on AHH activity in C2Rev7 cells. The results show that the hepatoma cells examined express to various degrees phenobarbital-inducible cytochrome P-450 and/or 3-methylcholanthrene-inducible cytochrome P-450. These cell lines are versatile tools for studying the regulation of monooxygenase activities and analysing their role in the activation and inactivation of xenobiotics such as carcinogens, drugs and pesticides.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/7-Alkoxycoumarin O-Dealkylase,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Aryl Hydrocarbon Hydroxylases,
http://linkedlifedata.com/resource/pubmed/chemical/Benz(a)Anthracenes,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Methylcholanthrene,
http://linkedlifedata.com/resource/pubmed/chemical/Mixed Function Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Phenobarbital,
http://linkedlifedata.com/resource/pubmed/chemical/aldrin epoxidase,
http://linkedlifedata.com/resource/pubmed/chemical/benz(a)anthracene
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0014-2956
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
17
|
| pubmed:volume |
145
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
455-62
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:6334605-7-Alkoxycoumarin O-Dealkylase,
pubmed-meshheading:6334605-Animals,
pubmed-meshheading:6334605-Antibodies, Monoclonal,
pubmed-meshheading:6334605-Aryl Hydrocarbon Hydroxylases,
pubmed-meshheading:6334605-Benz(a)Anthracenes,
pubmed-meshheading:6334605-Cell Line,
pubmed-meshheading:6334605-Cytochrome P-450 Enzyme System,
pubmed-meshheading:6334605-Dexamethasone,
pubmed-meshheading:6334605-Enzyme Induction,
pubmed-meshheading:6334605-Immunochemistry,
pubmed-meshheading:6334605-Liver Neoplasms, Experimental,
pubmed-meshheading:6334605-Male,
pubmed-meshheading:6334605-Methylcholanthrene,
pubmed-meshheading:6334605-Microsomes, Liver,
pubmed-meshheading:6334605-Mixed Function Oxygenases,
pubmed-meshheading:6334605-Oxygenases,
pubmed-meshheading:6334605-Phenobarbital,
pubmed-meshheading:6334605-Rats,
pubmed-meshheading:6334605-Rats, Inbred Strains
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Expression of cytochromes P-450 in rat hepatoma cells. Analysis by monoclonal antibodies specific for cytochromes P-450 from rat liver induced by 3-methylcholanthrene or phenobarbital.
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| pubmed:publicationType |
Journal Article
|