Linked Life Data

6333346

Source:http://linkedlifedata.com/resource/pubmed/id/6333346

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1984-12-17
pubmed:abstractText
Limiting-dilution culture of murine Ly-2+ T cells with concanavalin A (Con A) and irradiated spleen filler cells produces, with high efficiency, cytolytic T lymphocyte (CTL) clones. With most mouse strains (including CBA and C57BL/6) the specificity of these CTL clones drops after day 6 of culture, so that by day 9 the majority of clones can lyse most murine target cells, whether syngeneic or allogeneic. The rate of specificity degradation and relative target cell preference varies with the mouse strain. Some strains (e.g. BALB/c) do not show this effect and CTL clones remain specific to day 9. Many low natural killer (NK) cell strains (e.g. C57BL/6J.bg) maintain CTL specificity in such cultures, but the correlation between CTL specificity and NK status is not absolute. Growth of BALB/c precursor cells on CBA filler cells leads to specificity degradation in the BALB/c CTL clones; however, the specificity of CBA-derived CTL clones is not maintained by growth on BALB/c fillers. The results suggest that specificity degradation is induced in the developing CTL-clone by factors in the culture environment, perhaps a soluble lymphokine (a differentiation factor) or by an infectious agent (an endogenous mouse virus). Although such CTL specificity loss may render many limiting dilution studies of the CTL specificity repertoire invalid, the problem may be bypassed by an appropriate choice of mouse strain.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
951-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Degradation of specificity in cytolytic T lymphocyte clones: mouse strain dependence and interstrain transfer of nonspecific cytolysis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't