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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1984-11-9
|
| pubmed:abstractText |
Several factors significantly influenced lymphocyte response to PHA stimulation in standard cell culture systems. Optimal response was obtained with the lymphocyte concentration at about 1.5 X 10(6) cells. Cultures with or without serum did not differ greatly in PHA response at lower lymphocyte concentrations. However, serum was inhibitory at higher lymphocyte concentrations. Addition of erythrocytes (1-10 X 10(6)) was beneficial for lower cell concentrations but deleterious for higher concentrations. The temperature of incubation giving optimal PHA response was 41 degrees C.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0361-7742
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
161
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
151-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6333030-Animals,
pubmed-meshheading:6333030-Cell Count,
pubmed-meshheading:6333030-Chickens,
pubmed-meshheading:6333030-Erythrocytes,
pubmed-meshheading:6333030-Lymphocyte Activation,
pubmed-meshheading:6333030-Mitogens,
pubmed-meshheading:6333030-T-Lymphocytes,
pubmed-meshheading:6333030-Temperature
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Parameters influencing chicken T-cell response to mitogens.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro
|