Linked Life Data

6332253

Source:http://linkedlifedata.com/resource/pubmed/id/6332253

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-9-26
pubmed:abstractText
The addition of human C5a to antigen-stimulated cultures results in dose-dependent augmentation of the primary antibody response to antigen. The specificity of this effect is confirmed by the ability of C5ades Arg, but not of the structurally analogous C3a anaphylatoxin, to act in a parallel fashion. This effect appears to be mediated by interaction of C5a with cells of the monocytoid/macrophage lineage. These cells have been shown to bear surface receptors specific for C5a. In order to examine the role of soluble mediators in these events, culture supernatants were generated from splenic adherent cells or macrophage cell lines after exposure to a brief pulse of human C5a. Supernatants from the macrophage-like cell line P388D1, which bears surface receptors for C5a, enhance the PFC response to antigen, whereas those from the closely related cell line P388, which lacks surface receptors for C5a, fail to cause enhancement. Supernatants from splenic adherent cells, which also bear C5a receptors, similarly augment the SRBC response. Active supernatants, but not those devoid of activity, contain IL-1 activity. None of the supernatants contain IL-2 activity. These observations suggest that the recently described role of human C5a as an immunopotentiating modulator is mediated by its ability to induce production of IL-1 upon binding to specific receptors at the macrophage cell surface.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0277-6766
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7-10
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Monokine generation as a consequence of binding of human C5a anaphylatoxin to specific cell-surface receptors.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.