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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1984-8-22
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pubmed:abstractText |
To evaluate the mechanism of insulin action to increase rat 125I-labeled insulin-like growth factor II (125I-IGF-II) binding to rat adipocytes, we raised a potent rabbit antiserum against purified IGF-II receptors from rat placental membranes. The antiserum elicited a positive reaction at a 1:5000 dilution against purified IGF-II receptor in an ELISA and markedly inhibited 125I-IGF-II binding to adipocyte plasma membranes when added prior to the growth factor. Immunoprecipitation lines formed between agar plate wells containing antiserum versus IGF-II receptor, both in the presence and absence of 1 microM IGF-II, indicating that binding of anti-receptor Ig to the IGF-II receptor is not affected by occupancy of the IGF-II binding site. Intact adipocytes treated with or without insulin were incubated with anti-IGF-II receptor Ig, washed, and further incubated with 125I-labeled goat anti-rabbit IgG to monitor the amount of anti-receptor Ig bound. Insulin induced parallel increases in anti-IGF-II receptor Ig binding (2.4-fold) and 125I-IGF-II binding (3-fold) to the isolated cells. The dose-response relationship of insulin action on 125I-IGF-II binding and anti-receptor Ig binding was essentially identical with a half-maximal effect at approximately 0.07 nM insulin. That insulin does not act to expose new types of antigenic sites on IGF-II receptors was indicated by the demonstration that control adipocytes could readily adsorb the anti-receptor Ig. These data demonstrate that increased numbers of IGF-II receptors are displayed in an exposed position on the adipocyte cell surface in response to insulin.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-14169133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-364249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-4243733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-4659260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-5135623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-5890522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6134722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6257700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6258909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6263874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6267316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6268396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6279656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6286636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6300099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6304694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6305991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6307989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-658418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-668694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6754719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6771756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6938960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6989818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-6995478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-7007367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-7016879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6330732-78929
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Somatomedin,
http://linkedlifedata.com/resource/pubmed/chemical/Somatomedins
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4028-32
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:6330732-Adipose Tissue,
pubmed-meshheading:6330732-Animals,
pubmed-meshheading:6330732-Antigen-Antibody Complex,
pubmed-meshheading:6330732-Cell Membrane,
pubmed-meshheading:6330732-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:6330732-Immune Sera,
pubmed-meshheading:6330732-Immunodiffusion,
pubmed-meshheading:6330732-Insulin,
pubmed-meshheading:6330732-Kinetics,
pubmed-meshheading:6330732-Male,
pubmed-meshheading:6330732-Peptides,
pubmed-meshheading:6330732-Rats,
pubmed-meshheading:6330732-Rats, Inbred Strains,
pubmed-meshheading:6330732-Receptor, Insulin,
pubmed-meshheading:6330732-Receptors, Cell Surface,
pubmed-meshheading:6330732-Receptors, Somatomedin,
pubmed-meshheading:6330732-Somatomedins
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pubmed:year |
1984
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pubmed:articleTitle |
Insulin activates the appearance of insulin-like growth factor II receptors on the adipocyte cell surface.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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