Linked Life Data

6329479

Source:http://linkedlifedata.com/resource/pubmed/id/6329479

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-8-23
pubmed:abstractText
When maintained in some serum-containing media, fetal rat sympathetic neurons acquire substantial choline acetyltransferase activity and form cholinergic synapses in vitro. However, when they are maintained in a serum-free, defined culture medium, choline acetyltransferase activity is not detected and cholinergic synapses are not observed. In this study, we have examined the effects of various times of exposure to a medium inducing cholinergic function on the properties of neurons subsequently maintained in defined medium. We report that 2-day, but not 2-h, exposure to this inducing medium causes a long-lasting (greater than 6 weeks) increase (7-10-fold) in the activity of choline acetyltransferase and that, under these conditions, sympathetic neurons in vitro form cholinergic, electrical and mixed function cholinergic and electrical synapses. We conclude that a relatively brief exposure to media inducing cholinergic function can cause long-lasting changes in the functional properties of sympathetic neurons in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:volume
316
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-82
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Fetal rat sympathetic neurons maintained in a serum-free medium retain induced cholinergic characteristics.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.