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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1984-7-9
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pubmed:abstractText |
The effect of acyclovir on DNA synthesized in cells infected with herpes simplex virus type 1 was examined. DNA that was synthesized in infected cells in the presence of acyclovir during a short pulse with [3H]thymidine remained near the top of an alkaline sucrose gradient after centrifugation. The sedimentation characteristics of labeled DNA were not changed after chasing in isotope-free medium. The slowly sedimenting DNA was identified as viral in origin by hybridization to purified herpes simplex virus nucleocapsid DNA. When cells were infected with acyclovir-resistant virus containing mutations in the polymerase gene, the viral DNA synthesized in the presence of acyclovir was chased into high-molecular-weight DNA. These findings are consistent with chain termination of herpes simplex virus DNA in virus-infected cells.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0066-4804
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
507-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6329081-Acyclovir,
pubmed-meshheading:6329081-Animals,
pubmed-meshheading:6329081-Colony-Forming Units Assay,
pubmed-meshheading:6329081-Cricetinae,
pubmed-meshheading:6329081-DNA, Viral,
pubmed-meshheading:6329081-Drug Resistance, Microbial,
pubmed-meshheading:6329081-Kidney,
pubmed-meshheading:6329081-Nucleic Acid Hybridization,
pubmed-meshheading:6329081-Simplexvirus,
pubmed-meshheading:6329081-Virus Cultivation
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pubmed:year |
1984
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pubmed:articleTitle |
Identification of small DNA fragments synthesized in herpes simplex virus-infected cells in the presence of acyclovir.
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pubmed:publicationType |
Journal Article
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