Linked Life Data

6328561

Source:http://linkedlifedata.com/resource/pubmed/id/6328561

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-7-13
pubmed:abstractText
A catecholamine-sensitive adenylate cyclase activity was observed in cell homogenates of cultured renal epithelial (MDCK) cells. 10 microM isoprenaline gave a 2.85-fold increase in adenylate cyclase activity above basal levels. A series of adrenoceptor agonists gave a relative potency series of isoprenaline greater than adrenaline greater than noradrenaline (K1(9) values of 1.9 X 10(-7), 1.6 X 10(-6) and 1.9 X 10(-5) M respectively), consistent with activation of a beta-adrenoceptor. Intracellular accumulation of cyclic AMP was also stimulated by 10 microM isoprenaline, peak values being observed after 2 min, followed by a decline to lower maintained levels. The phosphodiesterase inhibitor isobutylmethylzanthine (1 mM) augmented isoprenaline-stimulated cyclic AMP accumulation. In epithelial preparations of MDCK cells grown upon Millipore filters and mounted in Ussing chambers isoprenaline was only effective in elevating intracellular cyclic AMP contents when applied to the basal cell surfaces. Direct measurement of beta-adrenoceptor density and subtype was determined by (+/-)-3-[125I]iodocyanopindolol binding to MDCK cell homogenates. Binding consisted of a saturable component (Vmax = 14.9 fmol/mg cell protein) of high molar affinity (Kd = 10.8 pM) and a non-saturable component which showed a linear dependence on iodocyanopindolol concentration. In addition to the high-affinity binding site, dissociation kinetics revealed a low-affinity component (Kd = 450 pM) comprising 24% of saturable binding. Competition of (+/-)-3-[125I]iodocyanopindolol binding with beta-adrenoceptor agonists and antagonists was entirely consistent with the existence of a beta 2-adrenoceptor. Examination of various MDCK cultures and clones revealed the existence of MDCK cultures whose adenylate cyclase activity was unresponsive to catecholamine stimulation; this correlated with a reduced or undetectable level of (+/-)-3-[125I]iodocyanopindolol binding. The control of transepithelial chloride transport in MDCK epithelia by catecholamines is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0144-8757
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
339-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Control of cultured epithelial (MDCK) cell transport function: identification of a beta-adrenoceptor coupled to adenylate cyclase.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't