Linked Life Data

6328314

Source:http://linkedlifedata.com/resource/pubmed/id/6328314

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5967
pubmed:dateCreated
1984-7-9
pubmed:abstractText
The sodium pump of animal cells is electrogenic, that is, it normally exports more sodium ions than it imports potassium ions. In the squid giant axon, the resulting net outward electric current has a density of a few microA cm-2, and contributes 1-2 mV to the resting membrane potential. The pump is driven by the free energy of hydrolysis of ATP, and in some instances it has been possible to run the pump backwards and synthesize ATP by lowering the [ATP]/[ADP] X [Pi] ratio and steepening the transmembrane Na+ and K+ gradients. Here we have examined the question of whether a backward-running sodium pump conserves its Na+/K+ greater than 1 stoichiometry. We demonstrate reversal of the sodium pump of squid giant axon, and find that backward pumping indeed produces a net inward electric current. This current is voltage-sensitive. Our observations have mechanistic implications for models of the sodium pump.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:volume
309
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
450-2
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Current generated by backward-running electrogenic Na pump in squid giant axons.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.