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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1984-7-18
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pubmed:abstractText |
The human T cell leukemia Jurkat was used as a model to examine the requirements of T cell activation. These studies demonstrated that antibodies reactive with the T cell-specific T3 antigen were insufficient to result in the activation of Jurkat cells, determined by the secretion of IL 2. IL 2 production occurred only in the presence of a second stimulus, the phorbol ester PMA. With the use of an IL 2-specific cDNA probe, the appearance of IL 2 RNA, similarly, occurred only when cells were stimulated with both anti-T3 antibodies and PMA. These results demonstrate a two-stimulus requirement for gene expression in human T cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
133
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
123-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6327821-Antibodies, Monoclonal,
pubmed-meshheading:6327821-Antigens, CD27,
pubmed-meshheading:6327821-Antigens, Surface,
pubmed-meshheading:6327821-Cell Line,
pubmed-meshheading:6327821-Humans,
pubmed-meshheading:6327821-Interleukin-2,
pubmed-meshheading:6327821-Leukemia,
pubmed-meshheading:6327821-Lymphocyte Activation,
pubmed-meshheading:6327821-Protein Biosynthesis,
pubmed-meshheading:6327821-RNA, Messenger,
pubmed-meshheading:6327821-T-Lymphocytes,
pubmed-meshheading:6327821-Tetradecanoylphorbol Acetate
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pubmed:year |
1984
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pubmed:articleTitle |
The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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