Linked Life Data

6327111

Source:http://linkedlifedata.com/resource/pubmed/id/6327111

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1984-7-13
pubmed:abstractText
Epidermal growth factor (EGF) interactions with primary epidermal cells in culture were examined in BALB/c and SENCAR mice, strains resistant and sensitive, respectively, to carcinogenesis by initiation-promotion. Using low (less than 0.1 mM) calcium growth conditions, which select for basal cells, and calcium-induced terminal differentiation, we determined the effects of retinoids, the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), and cell density on binding of 125I-labeled EGF. Over the range tested, EGF binding increased with increasing cell density similarly in basal cells of both strains, which at similar densities bound similar amounts of EGF. Increasingly differentiated epidermal cells of both strains bound less EGF. Responses of basal and differentiating cells were dissimilar in several respects. Most notably, differentiating cells bound but failed to metabolize EGF. TPA treatment of basal cells from either strain led to a rapid, pronounced reduction in EGF binding, while treatment with retinoic acid, an antipromoter in vivo, increased binding. In contrast, EGF binding by differentiating cells was much less affected by TPA treatment, and retinoic acid had no effect or was slightly inhibitory, while combined treatment was more inhibitory than either alone. Given an adequate plating density, inclusion of 1 ng EGF per ml of media significantly enhanced growth of basal cells. Because of the similarities in binding patterns between the two strains, it seems unlikely that differential responses of BALB/c and SENCAR epidermal cells to EGF and to modulators of EGF binding are involved in the differences in susceptibility of these strains to carcinogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
735-40
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Interaction of epidermal growth factor with basal and differentiating epidermal cells of mice resistant and sensitive to carcinogenesis.
pubmed:publicationType
Journal Article, Comparative Study