Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions |
umls-concept:C0018787,
umls-concept:C0027096,
umls-concept:C0027119,
umls-concept:C0034493,
umls-concept:C0035696,
umls-concept:C0040135,
umls-concept:C0205345,
umls-concept:C0220781,
umls-concept:C0392747,
umls-concept:C0441889,
umls-concept:C0443172,
umls-concept:C0851285,
umls-concept:C1420192,
umls-concept:C1883254
|
pubmed:issue |
8
|
pubmed:dateCreated |
1984-7-20
|
pubmed:abstractText |
The expression of mRNAs for two cardiac myosins has been examined in the ventricles of hypo- and hyperthyroid rabbits by means of cloned cDNA sequences corresponding to the mRNAs of the alpha- and beta-myosin heavy chains (HCs). The temporal change in the relative levels of the alpha- and beta-HC mRNAs after 3,5,3'-triiodothyronine (T3) treatment of hypothyroid rabbits was determined by nuclease S1 mapping. In the hypothyroid state, only HC beta-mRNA was expressed in the ventricles. The HC alpha-mRNA was first detectable 4 h after administration of T3 (200 micrograms/kg) to hypothyroid animals. By 12 h, HC alpha-mRNA represented 20% of total myosin mRNA, increasing to 50% by 24 h and to about 90% by 72 h. The relationship between the relative mRNA levels and relative synthesis rates of the myosin HCs was evaluated in 5-6-week-old normal and thyrotoxic rabbits. Myosin synthesis rates were determined by labeling of protein in vivo with [3H]leucine. The V1 (HC alpha) and V3 (HC beta) isomyosins were separated by affinity chromatography with monoclonal antibodies, and the HCs were isolated electrophoretically. In a normal euthyroid group of animals and in animals 12 and 24 h after administration of 200 micrograms of 3,5,3',5'-tetraiodothyronine/kg, the relative mRNA levels and relative synthesis rates of the alpha- and beta-HCs were not significantly different. Our results show that, first, thyroid hormone causes a rapid accumulation of HC alpha-mRNA and loss of HC beta-mRNA and, second, in normal and thyrotoxic rabbits, the relative synthesis rates of HC alpha and HC beta reflect the relative abundance of the alpha- and beta-HC mRNAs.(ABSTRACT TRUNCATED AT 250 WORDS)
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0006-2960
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
10
|
pubmed:volume |
23
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1596-9
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:6326804-Animals,
pubmed-meshheading:6326804-Base Sequence,
pubmed-meshheading:6326804-Cloning, Molecular,
pubmed-meshheading:6326804-DNA,
pubmed-meshheading:6326804-DNA Restriction Enzymes,
pubmed-meshheading:6326804-Hypothyroidism,
pubmed-meshheading:6326804-Kinetics,
pubmed-meshheading:6326804-Male,
pubmed-meshheading:6326804-Myocardium,
pubmed-meshheading:6326804-Myosins,
pubmed-meshheading:6326804-Protein Biosynthesis,
pubmed-meshheading:6326804-RNA, Messenger,
pubmed-meshheading:6326804-Rabbits
|
pubmed:year |
1984
|
pubmed:articleTitle |
Regulation of myosin synthesis by thyroid hormone: relative change in the alpha- and beta-myosin heavy chain mRNA levels in rabbit heart.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|