Linked Life Data

6325927

Source:http://linkedlifedata.com/resource/pubmed/id/6325927

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5963
pubmed:dateCreated
1984-6-20
pubmed:databankReference
pubmed:abstractText
The genetic basis of the antibody repertoire--estimated to exceed 10(6) different immunoglobulin molecules--is a major unanswered problem. The number of germ-line V kappa genes in the mouse genome is probably several hundred while the corresponding number for three out of four human V kappa subgroups (V kappa I, V kappa III and V kappa IV) is probably altogether only 15-20 (ref. 5). The kappa II proteins differ significantly in sequence from the other kappa-chain proteins. To determine the contribution of V kappa II genes to kappa-chain diversity, we searched for a human lymphoid cell line which produces a kappa II chain and report here for the first time the sequence of a V kappa II gene. According to blot hybridizations with this V kappa gene as a probe, subgroup II contributes about half as many genes to the V kappa gene repertoire as are detected by a V kappa I probe. Therefore the repertoire is rather small which implies that somatic mutations or other mechanisms must play an important role in the generation of light-chain diversity in humans.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:volume
309
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Contribution of human V kappa II germ-line genes to light-chain diversity.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't