Linked Life Data

6325365

Source:http://linkedlifedata.com/resource/pubmed/id/6325365

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1984-6-18
pubmed:abstractText
The goal of this experiment was to develop an in vitro model of HSV-1 infection and to characterize the virologic parameters associated with such an infection. An in vitro model of HSV-1 infection would offer a defined, efficient, and easily controlled system for studying the mechanisms associated with HSV-1 latency and reactivation. Results indicate that: (1) in the presence of 100 micrograms/ml acyclovir, acute infection is suppressed within 3 days; (2) during suppression, infectious virus was recovered only from whole cell trigeminal ganglion explants (no virus recovery from supernate or homogenized samples); immunofluorescent staining was evident with antiserum to VP175, but not with antiserum to HSV-1 and intranuclear inclusions, but no intact virions were observed in neurons by electron microscopy; (3) 72 hr after desuppression of HSV infected trigeminal ganglion cells infectious HSV-1 was recovered from supernate, homogenized, and whole cell cultures. Immunofluorescent staining was observed with antisera to VP175 and HSV-1; intranuclear inclusions as well as intact virus particles were noted in neurons via electron microscopy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0146-0404
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
525-33
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Suppression of HSV-1 infection in trigeminal ganglion cells. An in vitro model of latency.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't