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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1984-6-20
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pubmed:abstractText |
Two size classes of O-glycosidically linked oligosaccharides were liberated from glycoprotein E1 of mouse hepatitis virus (MHV) A59 by reductive beta-elimination and separated by h.p.l.c. The structures of the reduced oligosaccharides were determined by successive exoglycosidase digestions and by methylation analyses involving combined capillary gas chromatography-mass spectrometry and mass fragmentography after chemical ionization with ammonia. Oligosaccharide A (Neu5Ac alpha 2----3 Gal beta 1----3 GalNAc) comprised 35% of the total carbohydrate side chains, while the remaining 65% of the oligosaccharides of E1 had the branched structure B: Neu5Ac alpha 2----3 Gal beta 1----3 (Neu5Ac alpha 2----6) GalNAc. Both oligosaccharides were linked to the E1 polypeptide via N-acetylgalactosamine, and 20% of the sialic acids present in E1 glycopeptides were found to consist of N-acetyl-9-mono-O-acetylneuraminic acid. The reported structures of the O-linked glycans are discussed in the context of the amino acid sequence of E1, which exhibits a cluster of four hydroxyamino acids (Ser-Ser-Thr-Thr) as potential O-glycosylation sites at the amino terminus. Oligosaccharides with identical structures and an identical O-glycosylated tetrapeptide sequence are present in the blood group M-active glycophorin A of the human erythrocyte membrane.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-14135466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-4270451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-5333200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-5350948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-5637714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-6245243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-6264128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-6275093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-6281976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-6283141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-6299584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-6327272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-6638480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-7233832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-7273037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-7276911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-728384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-7314449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-7343686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-7396920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6325180-87397
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
665-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1984
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pubmed:articleTitle |
The carbohydrates of mouse hepatitis virus (MHV) A59: structures of the O-glycosidically linked oligosaccharides of glycoprotein E1.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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