|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1984-5-2
|
|
pubmed:abstractText |
DNA prepared from ouabain-resistant mouse cells was able to transform ouabain-sensitive CV-1 cells to ouabain resistance after DNA-mediated gene transfer. The murine DNA fragment responsible for ouabain resistance was detected on the background of CV-1 DNA by virtue of a repetitive DNA sequence element that reacts positively with a mouse repeat DNA clone. CV-1 DNA is nonreactive with this probe. Southern analysis of several independently derived ouabain-resistant transformants indicates that the mouse ouaR gene is located on a 6.5-kilobase EcoRI restriction fragment. The 6.5-kilobase DNA fragment was initially isolated from a lambda phage library made from a ouabain-resistant secondary transformant and subsequently was subcloned in the plasmid vector pAT153. This plasmid was able to transform wild-type CV-1 cells to ouabain resistance at a frequency of about 10 cells per ng of DNA.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-14240177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-284385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-286319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-373120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-4559594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-6246445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-6251468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-6266915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-6272391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-6286138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-6865947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-7292261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-7460013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324196-881736
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
81
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1489-93
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:6324196-Animals,
pubmed-meshheading:6324196-Base Sequence,
pubmed-meshheading:6324196-Cell Line,
pubmed-meshheading:6324196-Cercopithecus aethiops,
pubmed-meshheading:6324196-Cloning, Molecular,
pubmed-meshheading:6324196-DNA, Recombinant,
pubmed-meshheading:6324196-Drug Resistance,
pubmed-meshheading:6324196-Genes,
pubmed-meshheading:6324196-Lung,
pubmed-meshheading:6324196-Mice,
pubmed-meshheading:6324196-Nucleic Acid Hybridization,
pubmed-meshheading:6324196-Ouabain,
pubmed-meshheading:6324196-Plasmids,
pubmed-meshheading:6324196-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:6324196-Transfection
|
|
pubmed:year |
1984
|
|
pubmed:articleTitle |
Molecular cloning of the mouse ouabain-resistance gene.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
