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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1984-5-2
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pubmed:abstractText |
We determined that mouse lymphoid cell lines can be transfected at high efficiencies (10-70%) by a polyoma virus shuttle vector. With this vector, we obtained expression of a cloned mouse alpha heavy chain gene transfected into cell lines representative of all stages in B-lymphocyte development, a T-cell lymphoma line, and 3T3 fibroblasts. Heavy chain gene expression in transfected light chain-producing myeloma cells occurred at levels comparable to those in IgA-secreting myeloma cells. Heavy chains produced in transfected myeloma cells were associated with light chains in membrane-bound IgA. While T-lymphoma cells and fibroblasts were transfected at similar efficiencies to B cells, significantly lower levels of alpha heavy chains were produced. This immunoglobulin gene transfection system provides a powerful approach for defining important regulatory regions in immunoglobulin genes and for identifying lymphoid cell factors involved in immunoglobulin gene expression in B-lymphocyte development.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-225042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-226716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-301614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-308074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-319344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-370836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-5305881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6181924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6248242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6263759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6277500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6277501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6278479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6280876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6300842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6402777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6403868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6404604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6409417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6409418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6409419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6416835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6443558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6443559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6766532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6771019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6801653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-6818555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-789096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6324184-825377
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1292-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6324184-Animals,
pubmed-meshheading:6324184-Cell Line,
pubmed-meshheading:6324184-Cloning, Molecular,
pubmed-meshheading:6324184-DNA Restriction Enzymes,
pubmed-meshheading:6324184-Genes,
pubmed-meshheading:6324184-Genetic Vectors,
pubmed-meshheading:6324184-Immunoglobulin A,
pubmed-meshheading:6324184-Immunoglobulin Heavy Chains,
pubmed-meshheading:6324184-Lymphocytes,
pubmed-meshheading:6324184-Lymphoma,
pubmed-meshheading:6324184-Mice,
pubmed-meshheading:6324184-Polyomavirus,
pubmed-meshheading:6324184-Transfection
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pubmed:year |
1984
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pubmed:articleTitle |
Expression of an immunoglobulin heavy chain gene transfected into lymphocytes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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