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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1984-5-24
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pubmed:abstractText |
Recombinant DNA molecules consisting of the simian virus 40 (SV40) early region and different subgenomic hepatitis B virus DNA fragments were constructed in vitro and packaged in vivo into SV40 capsids by using a complementing SV40 helper virus. Upon infection with these virus stocks the three known hepatitis B-specific antigens were expressed under SV40 control. The surface antigen was released into the medium, and the core antigen and its derivative hepatitis B e antigen were only detected intracellularly. Size analysis of the core gene product(s) by immunoblotting revealed the presence of a single protein species identical with the 21-kilodalton core antigen isolated from human liver. The hepatitis B core antigen expressing construct did not contain a putative precore sequence, indicating that such a sequence is not needed for hepatitis B core antigen synthesis in animal cells. S1 analysis demonstrated the use of SV40 signals for initiation and polyadenylation of the core gene transcripts. In addition, a processing-polyadenylation signal was identified within the core gene.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6323734-105041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6323734-11894903,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6323734-171410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6323734-187208,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6323734-6172555,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6323734-822353
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
335-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6323734-Cell Line,
pubmed-meshheading:6323734-DNA, Recombinant,
pubmed-meshheading:6323734-DNA Restriction Enzymes,
pubmed-meshheading:6323734-Genetic Vectors,
pubmed-meshheading:6323734-Hepatitis B Antigens,
pubmed-meshheading:6323734-Hepatitis B Surface Antigens,
pubmed-meshheading:6323734-Hepatitis B e Antigens,
pubmed-meshheading:6323734-Humans,
pubmed-meshheading:6323734-Kinetics,
pubmed-meshheading:6323734-Nucleic Acid Hybridization,
pubmed-meshheading:6323734-Plasmids,
pubmed-meshheading:6323734-Simian virus 40,
pubmed-meshheading:6323734-Transcription, Genetic
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pubmed:year |
1984
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pubmed:articleTitle |
Expression of hepatitis B antigens with a simian virus 40 vector.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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