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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1984-5-24
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pubmed:abstractText |
Messenger RNA was prepared from a hybridoma cell line secreting a monoclonal antibody (53FC3) directed against a luminal epitope of a Golgi membrane protein (Mr = 135 kd) found in rodent cells. When this mRNA was microinjected into the cytoplasm of BHK cells, mouse IgG was seen to accumulate in the Golgi complex after 5-6 hr of incubation. No accumulation was seen in 3T3 cells which lack the epitope recognized by 53FC3. When microinjected BHK cells were infected with vesicular stomatitis virus, surface expression of the viral G protein was considerably reduced when compared with neighboring noninjected cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
847-56
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:6323023-Animals,
pubmed-meshheading:6323023-Antibodies, Monoclonal,
pubmed-meshheading:6323023-Cell Line,
pubmed-meshheading:6323023-Cells, Cultured,
pubmed-meshheading:6323023-Cricetinae,
pubmed-meshheading:6323023-Cricetulus,
pubmed-meshheading:6323023-Fluorescent Antibody Technique,
pubmed-meshheading:6323023-Golgi Apparatus,
pubmed-meshheading:6323023-Hybridomas,
pubmed-meshheading:6323023-Kidney,
pubmed-meshheading:6323023-Membrane Glycoproteins,
pubmed-meshheading:6323023-Membrane Proteins,
pubmed-meshheading:6323023-Mice,
pubmed-meshheading:6323023-Microinjections,
pubmed-meshheading:6323023-RNA, Messenger,
pubmed-meshheading:6323023-Vesicular stomatitis Indiana virus,
pubmed-meshheading:6323023-Viral Envelope Proteins,
pubmed-meshheading:6323023-Viral Proteins
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pubmed:year |
1984
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pubmed:articleTitle |
Microinjection of mRNA coding for an anti-Golgi antibody inhibits intracellular transport of a viral membrane protein.
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pubmed:publicationType |
Journal Article
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