Linked Life Data

6321998

Source:http://linkedlifedata.com/resource/pubmed/id/6321998

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5953
pubmed:dateCreated
1984-4-2
pubmed:abstractText
The expression of a number of eukaryotic genes has been shown to involve at least two sequences located upstream of the actual transcription unit: one of these sequences, centred on a widely conserved TATAAT sequence, is thought to be involved in determining the precise site of initiation of transcription; the other has a gene-specific sequence, can function at a variable distance upstream of the initiation site, and is involved in the regulation of transcription. By constructing beta-galactosidase gene fusions, to facilitate measuring gene expression in vivo, we have now defined a cis-acting regulatory element of the Saccharomyces cerevisiae leu2 gene. This element is located within a 280 base pair (bp) fragment which occurs 125 bp upstream of the leu2 translation initiation codon and which contains a short G + C-rich palindromic sequence. A fragment of the Escherichia coli transposable element Tn9 which contains a similar palindromic sequence can functionally replace the natural leu2 regulatory element. Our results are contrary to previous speculations that the leu2 gene is regulated by an attenuation mechanism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:volume
307
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
740-2
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Role of an upstream regulatory element in leucine repression of the Saccharomyces cerevisiae leu2 gene.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.