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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1984-4-26
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pubmed:abstractText |
We previously described infectious retrovirus vectors constructed from spleen necrosis virus which contain the herpes simplex virus thymidine kinase gene and the mouse alpha-globin gene (K. Shimotohno and H. M. Temin, Nature [London] 299:255-268, 1982). In the present study we report that when TK- chicken cells infected with a virus containing the mouse alpha-globin promoter and other 5' noncoding sequences in addition to the alpha-globin coding sequences were selected for thymidine kinase (TK) activity, all virus-producing TK+ cell clones shed virus with a deletion. These deletions were of different sizes and included the mouse alpha-globin coding sequences and the mouse alpha-globin transcriptional promoter. One of the deleted viruses was molecularly cloned. DNA sequencing showed that the deleted sequences are flanked by a short direct repeat. This deleted virus was also shown to have an advantage over the nondeleted parent both in multiplication and in its specific TK-transforming unit titer. In contrast to the results described above, TK+ cell clones established with viruses that contained only the coding sequences from the mouse alpha-globin gene did not delete and were stable over many cell passages. The implications of the high-frequency deletion of the viruses with internal promoters are discussed in terms of the evolution of retroviruses and the construction of retrovirus vectors.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-372543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-4332983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-519760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6256659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6258798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6262799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6268304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6273733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6276009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6283110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6287291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6294985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6297744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6297766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6304690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6305507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6308421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6312073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6572371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6574469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-6985477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-7063411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6321798-847462
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
42-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6321798-Animals,
pubmed-meshheading:6321798-Base Sequence,
pubmed-meshheading:6321798-Cell Line,
pubmed-meshheading:6321798-Chickens,
pubmed-meshheading:6321798-Chromosome Deletion,
pubmed-meshheading:6321798-Clone Cells,
pubmed-meshheading:6321798-DNA, Recombinant,
pubmed-meshheading:6321798-Genes, Viral,
pubmed-meshheading:6321798-Genetic Vectors,
pubmed-meshheading:6321798-Globins,
pubmed-meshheading:6321798-Operon,
pubmed-meshheading:6321798-Retroviridae,
pubmed-meshheading:6321798-Thymidine Kinase,
pubmed-meshheading:6321798-Transfection,
pubmed-meshheading:6321798-Virus Replication
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pubmed:year |
1984
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pubmed:articleTitle |
High-frequency deletion in recovered retrovirus vectors containing exogenous DNA with promoters.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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