Restriction fragment length polymorphisms have been identified in the alpha-globin pseudogene carried on chromosome 17. Different inbred strains of mice carry one of two forms of the sequence, whereas all complete t haplotypes carry a third form. With the use of partial t haplotypes, the alpha-psi 4 sequence has been mapped to the distal end of the t complex. This sequence appears to be closely linked to the tufted locus in the t complex.