Linked Life Data

6320005

Source:http://linkedlifedata.com/resource/pubmed/id/6320005

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5950
pubmed:dateCreated
1984-3-9
pubmed:abstractText
The previous demonstration of an increase and redistribution of adenylate cyclase activity in injured peripheral nerve suggests that an increase in neuronal cyclic AMP concentration could play a role in peripheral nerve regeneration. We report our finding that accumulating adenylate cyclase activity was translated into a twofold increase in cyclic AMP concentration in the regenerating nerve stump, coincident with the initiation and elongation of regenerative nerve sprouts. We sought to magnify the role of cyclic AMP in regeneration by using forskolin, a robust activator of adenylate cyclase, to produce an additional increase in neuronal cyclic AMP in situ. Forskolin in vitro produced an approximately 40-fold greater elevation in neuronal cyclic AMP than an equimolar (10(-5] concentration of isoprenaline. Moreover, the elevated cyclic AMP concentration persisted for at least 60 min in the continued presence of forskolin. Daily injection of forskolin into the dorsal lymph sac of Rana pipiens, or delivery of forskolin through an implanted osmotic pump produced a sustained 40% increase in the rate of sensory nerve regeneration in freeze-lesioned sciatic nerves. We conclude that an increase in cyclic AMP concentration and, presumably, the activation of appropriate protein kinases stimulates regenerative nerve growth following trauma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:volume
307
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
455-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:articleTitle
Forskolin activation of adenylate cyclase in vivo stimulates nerve regeneration.
pubmed:publicationType
Journal Article