Linked Life Data

6318394

Source:http://linkedlifedata.com/resource/pubmed/id/6318394

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1984-2-14
pubmed:abstractText
Lesions were induced in the plasma membranes of cultured human fibroblasts by membrane damaging toxins of bacterial origin (haemolysins). Structural disorganization of the membrane was measured as leakage of a radiolabelled small cytoplasmic marker and functional membrane damage was measured as decreased uptake of aminoisobutyrate. Cell survival was scored 24 and 48 hr later by measuring uptake of Trypan Blue and by light microscopical evaluation of cell morphology and proliferation. The membrane damage induced by most bacterial toxins was reversible upon removal of the toxin, since toxin-treated cells recovered and excluded Trypan Blue although they had been permeable to the dye immediately after the toxin treatment. Among ten bacterial toxins tested, the only exception of this general behavior was the Aeromonas hydrophila beta-haemolysin, which irreversibly damaged human fibroblasts. Thus, the action of bacterial haemolysins on cultured cells generally seems restricted to a plasma membrane permeabilization, which is reversible regardless of the mechanism of membrane damaging action of the toxin or of the relative size of the structural lesions induced. Furthermore, the use of Trypan Blue uptake as a measure of cell death caused by membrane damaging agents appears to be of limited value.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0041-0101
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
805-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Survival of cultured cells after functional and structural disorganization of plasma membrane by bacterial haemolysins and phospholipases.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't