Linked Life Data

6318392

Source:http://linkedlifedata.com/resource/pubmed/id/6318392

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1984-2-15
pubmed:abstractText
Isolated rat adrenal cells in tissue culture, showing ACTH-induced corticosterone synthesis, were used as a model system for the study of adrenal metabolism and toxicity of 7,12-dimethylbenz[a]anthracene (DMBA). DMBA was metabolized at a rate of 10 pmol/min/10(6) cells and with a Km of 0.5 microM. Metabolite patterns and sensitivity to various AHH inhibitors suggest the involvement of an epoxide intermediate. In agreement with this proposal DMBA metabolite(s) were bound to cellular protein at a rate which was related to the AHH activity. Adrenal AHH was found to be insensitive to ACTH during a period of 24 h. Using ACTH-induced corticosterone synthesis as an indicator of cell damage the hepatic metabolite 7-hydroxymethyl-12-methyl-benz[a]anthracene was shown to be significantly more toxic than the parent compound DMBA. It is concluded that DMBA-dependent adrenal damage in vivo is due mainly to the liver metabolite 7-hydroxymethyl-12-methyl-benz[a]anthracene (7-OHM-12-MBA), possibly after secondary metabolic activation in the adrenal.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0300-483X
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-59
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Metabolism and toxic effects of 7,12-dimethyl-benz[a]anthracene in isolated rat adrenal cells.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't