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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1984-2-14
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pubmed:abstractText |
The amplified CAD genes in N-(phosphonacetyl)-L-aspartate (PALA)-resistant Syrian hamster cells are located in an expanded chromosomal region emanating from the site of the wild-type gene at the tip of the short arm of chromosome B-9. The terminus of B-9 in PALA-sensitive cells contains a cluster of rRNA genes (i.e., a nucleolus organizer, rDNA). We have used a molecular clone containing sequences complementary to Syrian hamster 28S rRNA to investigate whether rDNA is coamplified with CAD genes in the PALA-resistant mutants. In situ hybridization of this probe to metaphase chromosomes demonstrates that rDNA and CAD genes do coamplify in two independently isolated PALA-resistant mutants. The tight linkage of CAD and rDNA genes was demonstrated by their coordinate translocation from B-9 to the end of the long arm of chromosome C-11 in one mutant. Blot hybridization studies substantiate the in situ hybridization results. Both types of analysis indicate that only one or two rDNA genes, on the average, are coamplified per CAD gene. The data are consistent with the model that unequal exchanges between rDNA genes mediate the amplification of CAD genes in the Syrian hamster mutants that were analyzed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-12870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-183818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-281704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-284385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-334045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-338920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-381311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-4819731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-4894690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-5538845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6125880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6165993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6180304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6256084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6260574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6272316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6273843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6277500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6282856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6298598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6775816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6789329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6928643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6934533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6952245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-6987539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-7328649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-7363327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-845942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-881736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6318080-975935
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aspartate Carbamoyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/CAD trifunctional enzyme,
http://linkedlifedata.com/resource/pubmed/chemical/Carbamoyl-Phosphate Synthase...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydroorotase,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/NSC 224131,
http://linkedlifedata.com/resource/pubmed/chemical/Organophosphorus Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphonoacetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2066-75
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6318080-Animals,
pubmed-meshheading:6318080-Aspartate Carbamoyltransferase,
pubmed-meshheading:6318080-Aspartic Acid,
pubmed-meshheading:6318080-Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing),
pubmed-meshheading:6318080-Cell Line,
pubmed-meshheading:6318080-Chromosomes,
pubmed-meshheading:6318080-Cricetinae,
pubmed-meshheading:6318080-DNA Restriction Enzymes,
pubmed-meshheading:6318080-Dihydroorotase,
pubmed-meshheading:6318080-Drug Resistance,
pubmed-meshheading:6318080-Gene Amplification,
pubmed-meshheading:6318080-Genes,
pubmed-meshheading:6318080-Mesocricetus,
pubmed-meshheading:6318080-Models, Genetic,
pubmed-meshheading:6318080-Multienzyme Complexes,
pubmed-meshheading:6318080-Nucleic Acid Hybridization,
pubmed-meshheading:6318080-Organophosphorus Compounds,
pubmed-meshheading:6318080-Phosphonoacetic Acid,
pubmed-meshheading:6318080-Proteins,
pubmed-meshheading:6318080-RNA, Ribosomal,
pubmed-meshheading:6318080-Sister Chromatid Exchange
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pubmed:year |
1983
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pubmed:articleTitle |
Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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