Linked Life Data

6316807

Source:http://linkedlifedata.com/resource/pubmed/id/6316807

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-1-27
pubmed:abstractText
An ultramicro phospho-L-arginine (Arg-P) assay for nanoliter-volume biological samples is described. The assay and method is demonstrated to be reliable and reproducible for the detection of subpicomole quantities of both Arg-P and ATP in serial samples of cytoplasm from a single, isolated giant nerve fiber from the crayfish Procambarus clarkii. The assay for Arg-P is based on the ATP:L-arginine Nw-phosphotransferase (EC 2.7.3.3.)-catalyzed phosphorylation of ADP to ATP. The generated ATP is measured with a standard luciferin-luciferase bioluminescence method. Arg-P and ATP concentrations of pure axoplasmic samples from resting nerve were found to be approximately 13 and 2 mM, respectively. There was no evidence that substances present in the biological sample interfered with the assay. Recovery of added Arg-P and ATP were approximately 100%. Arg-P and ATP concentrations of isolated biological samples were found to be stable for 1 h at room temperature. The techniques described here are useful for the study of energy metabolism in single, isolated giant cells, and could be easily modified for the study of a variety of metabolic intermediates in situ in functioning tissues and organs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
134
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
413-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Enzymatic determination of subpicomole quantities of phospho-L-arginine in nerve cytoplasm.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.