Linked Life Data

6316436

Source:http://linkedlifedata.com/resource/pubmed/id/6316436

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-1-27
pubmed:abstractText
Washed rat peritoneal cells (RPC) rapidly and efficiently incorporated exogenous [3H]-arachidonic acid (AA). Exposure of labeled RPC to the calcium ionophore A23187 induced production of [3H]-leukotriene C4, D4 and E4 (LTC4, LTD4 and LTE4) and [3H]-prostaglandins (PGs). The radiolabeled lipoxygenase metabolites were isolated by a combination of organic extraction, silicic acid chromatography and reverse-phase high pressure liquid chromatography (RP-HPLC). Authentic leukotrienes (LT) were used to identify the biologically-synthesized products on RP-HPLC and to calculate recovery. The endogenously generated LT were also characterized by their ability to contract guinea-pig ileum and their susceptibility to soybean lipoxygenase. A23187 induced a bell-shaped concentration-dependent release of [3H]-LT which peaked at 1 X 10(-6)M ionophore. Kinetic studies revealed that ionophore stimulated the formation of mainly [3H]-LTE4 with only transient accumulation of [3H]-LTC4 and D4. In the presence of cysteine, however, production of [3H]-LTE4 was abolished with a subsequent accumulation of [3H]-LTD4. Non-steroidal antiinflammatory drugs (NSAID) moderately enhanced A23187-induced [3H]-LT production, whereas phospholipase and lipoxygenase inhibitors inhibited. The method is sensitive, reliable, and efficient for measurement of small quantities of LT produced from isolated cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0034-5164
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3-23
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Development of a sensitive method for measurement of leukotriene production by isolated cells.
pubmed:publicationType
Journal Article