Linked Life Data

6315507

Source:http://linkedlifedata.com/resource/pubmed/id/6315507

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1984-1-7
pubmed:abstractText
Growth response of mammary epithelial cells to hormones, particularly to prolactin, was studied by using a primary culture of rat mammary gland organoids. After allowing the cells to attach and spread on the surface of plastic culture dishes, the effect of hormones was tested by means of [3H]-thymidine incorporation and autoradiography in a medium containing 1% fetal calf serum. In this system, prolactin showed a modest but significant growth-stimulatory activity (50% over control), and addition of insulin or hydrocortisone or both enhanced the growth stimulation of prolactin to a large extent. Growth stimulation caused by these hormones without prolactin was always significantly lower than that caused with prolactin. A dose-response study indicated that prolactin can stimulate growth at physiological concentrations. The maximum stimulation was observed at 1-5 micrograms/ml. The growth-stimulatory effect of prolactin was decreased as the culture period was prolonged, and by the 4th day in culture the effect was no longer observed. In contrast, the stimulatory effect of insulin was constant over the 5-day culture period. Phosphoethanolamine, which has been shown to be a growth factor for some rat and human mammary carcinoma cells, showed 2-fold growth stimulation when added with prolactin, insulin or hydrocortisone. The stimulatory effect was again not observed in older cultures, as in the case of prolactin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-70
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Growth responsiveness to prolactin and its loss in normal rat mammary cells in culture.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't