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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1984-1-7
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pubmed:abstractText |
The studies presented herein describe the usefulness of glycerol in enhancing the binding activity and stability of detergent-solubilized gonadotropin (LH/hCG) receptors. The data presented show that when Leydig tumor cells (designated MA-10) are extracted with Triton X-100 in the presence of glycerol, one can quantitatively solubilize the cellular LH/hCG receptors without losing hormone-binding activity. It is also shown that in the presence of glycerol, the free soluble receptors can be maintained for at least 3 days with little or no reduction in their hormone-binding activity. These findings should prove useful for future studies on the structure and properties of gonadotropin receptors.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0013-7227
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
113
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2129-34
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6315342-Animals,
pubmed-meshheading:6315342-Cell Line,
pubmed-meshheading:6315342-Chorionic Gonadotropin,
pubmed-meshheading:6315342-Drug Stability,
pubmed-meshheading:6315342-Glycerol,
pubmed-meshheading:6315342-Iodine Radioisotopes,
pubmed-meshheading:6315342-Leydig Cell Tumor,
pubmed-meshheading:6315342-Methods,
pubmed-meshheading:6315342-Receptors, Cell Surface,
pubmed-meshheading:6315342-Receptors, LH,
pubmed-meshheading:6315342-Solubility
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pubmed:year |
1983
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pubmed:articleTitle |
An improved method for the solubilization of stable gonadotropin receptors.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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