Linked Life Data

6315208

Source:http://linkedlifedata.com/resource/pubmed/id/6315208

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1984-1-7
pubmed:abstractText
Running exercise trained rats at either 60 or 76% of their VO2max caused myocardial cyclic AMP phosphodiesterase (PDE) activity to be increased above control levels for at least 24 h following work. Neither training nor the exercise had any effect on the total concentration of calmodulin in heart tissues. The affinity of PDE for cyclic AMP was not changed by the exercise or training. The chelating agent, EGTA, had the same influence on PDE activity regardless of whether it was present in assays of control or exercised heart extract. Km and EGTA results suggest that calcium-bound calmodulin does not account for the higher PDE activity in the hearts of exercised rats. Supernatants from hearts homogenized in the presence of charcoal, to remove nucleotides from the extract, did not eliminate the exercise-associated increase in PDE activity. These results suggest that the elevated activity was not caused by an in vitro nucleotide activation. Preincubation of the enzyme from exercised and control rat hearts with snake venom activated PDE when assays were performed with the low concentration of cyclic AMP (1 microM). Moreover, the activity reached in the extract of exercisers (23.3 pmol X 100 microL-1 X min-1) was significantly greater than the activity found in control hearts (17.59 pmol X 100 microL-1 X min-1). Exercise increases PDE activity in the myocardium of trained rats.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-4212
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1017-24
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Cyclic AMP phosphodiesterase activity in the hearts of trained rats.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't