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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1983-12-17
|
| pubmed:abstractText |
Secretory granules, isolated from bovine neurohypophyses on isoosmolar Percoll-sucrose-EGTA gradients had a calmodulin content of 0.09 +/- 0.01 micrograms/mg protein (SE, n = 6). The distribution of calmodulin on the gradient showed that it did not copurify with the granules. Specific binding sites for calmodulin with a high affinity (Kd = 2.43 +/- 0.27 X 10(-9) M (SE, n = 5] and a maximum binding capacity of 1.3 +/- 0.4 pmol/mg protein (SE, n = 5) could be demonstrated when such secretory granules were incubated with 125I-calmodulin.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0001-6772
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
118
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
355-9
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6314746-Animals,
pubmed-meshheading:6314746-Binding Sites,
pubmed-meshheading:6314746-Calmodulin,
pubmed-meshheading:6314746-Calmodulin-Binding Proteins,
pubmed-meshheading:6314746-Cattle,
pubmed-meshheading:6314746-Cytoplasmic Granules,
pubmed-meshheading:6314746-Phosphoprotein Phosphatases,
pubmed-meshheading:6314746-Pituitary Gland, Posterior
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Calmodulin binding to secretory granules isolated from bovine neurohypophyses.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|