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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
21
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pubmed:dateCreated |
1983-12-17
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pubmed:databankReference | |
pubmed:abstractText |
The nucleotide sequence of the cytochrome oxidase subunit 2 (COX2) gene has been obtained from cloned mitochondrial DNA segments of Neurospora crassa. The coding sequences have been identified on the basis of protein sequence homology with the subunit 2 of cytochrome oxidase from yeast and man. The postulated precursor of the N. crassa subunit 2 protein is 250 amino acids long, with a molecular weight of 28,700. As in the tRNA and rRNA genes, the subunit 2 gene is flanked by G + C-rich palindromic sequences, which are highly conserved in N. crassa mitochondria. Three major transcripts have been detected by Northern blot hybridization. A transcript of 1100 bases is tentatively considered the fully processed mRNA. Furthermore, S1 nuclease protection experiments have revealed that the putative subunit 2 mRNA has a 330 nucleotide long 5' leader sequence.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
258
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13230-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6313689-Amino Acid Sequence,
pubmed-meshheading:6313689-Base Sequence,
pubmed-meshheading:6313689-Cloning, Molecular,
pubmed-meshheading:6313689-DNA, Mitochondrial,
pubmed-meshheading:6313689-DNA Restriction Enzymes,
pubmed-meshheading:6313689-Electron Transport Complex IV,
pubmed-meshheading:6313689-Genes,
pubmed-meshheading:6313689-Genes, Fungal,
pubmed-meshheading:6313689-Humans,
pubmed-meshheading:6313689-Neurospora,
pubmed-meshheading:6313689-Neurospora crassa,
pubmed-meshheading:6313689-Nucleic Acid Conformation,
pubmed-meshheading:6313689-Nucleic Acid Hybridization,
pubmed-meshheading:6313689-Saccharomyces cerevisiae,
pubmed-meshheading:6313689-Species Specificity
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pubmed:year |
1983
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pubmed:articleTitle |
Cytochrome oxidase subunit 2 gene in Neurospora crassa mitochondria.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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