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rdf:type |
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lifeskim:mentions |
umls-concept:C0013879,
umls-concept:C0017262,
umls-concept:C0038975,
umls-concept:C0086860,
umls-concept:C0185117,
umls-concept:C0205087,
umls-concept:C0376702,
umls-concept:C0442335,
umls-concept:C1511790,
umls-concept:C2603343,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
1983-12-20
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pubmed:abstractText |
Plasmids containing hepatitis B virus (HBV) DNA and a 232-bp SV40 DNA fragment encoding the origin of replication were constructed. When introduced by transfection into COS cells, these plasmids directed the synthesis of hepatitis B surface antigen. S1 mapping of the mRNAs covering the S gene showed that transcriptional initiation was promoted by the interaction of HBV sequences with an SV40 promoter element: transcription started on HBV DNA but had several properties of SV40 late transcription. The detection of a promoter element in an SV40 origin fragment commonly used in the COS system is important for the interpretation of data deriving from expression studies in COS cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-1060140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-218021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-377294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-383387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-390497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-399329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6256739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6260373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6264427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6264599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6267479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6277501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6278479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6279878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-6292733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-7063411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-7086959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-7121604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-7304971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6313342-7414320
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
511-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6313342-Animals,
pubmed-meshheading:6313342-Cell Line,
pubmed-meshheading:6313342-DNA, Recombinant,
pubmed-meshheading:6313342-DNA, Viral,
pubmed-meshheading:6313342-Gene Expression Regulation,
pubmed-meshheading:6313342-Genetic Vectors,
pubmed-meshheading:6313342-Hepatitis B Surface Antigens,
pubmed-meshheading:6313342-Hepatitis B virus,
pubmed-meshheading:6313342-Operon,
pubmed-meshheading:6313342-Plasmids,
pubmed-meshheading:6313342-Simian virus 40
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pubmed:year |
1983
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pubmed:articleTitle |
Detection of an element of the SV40 late promoter in vectors used for expression studies in COS cells.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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