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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1983-12-17
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pubmed:databankReference |
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pubmed:abstractText |
Activation of some variant surface glycoprotein (VSG) genes involves a duplicative transposition to an expression site, which completes the gene by addition of a mini-exon coding for the 5' 35 nucleotides of VSG mRNAs. Using a 22 nucleotide probe we have found some 200 copies of the mini-exon on a tandemly arranged 1.35 kb repetitive element. This repeat is highly conserved in three trypanosome species. The mini-exon on the repeat is flanked by a 5' splice site that resembles the consensus sequence. We have not found a single mini-exon within 10 kb of the transposed VSG gene exon in the expression site. We propose a model in which the arrays of mini-exon repeats function as a repetitive promoter for efficient transcription of VSG genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
891-900
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pubmed:dateRevised |
2008-8-29
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pubmed:meshHeading |
pubmed-meshheading:6313212-Animals,
pubmed-meshheading:6313212-Base Sequence,
pubmed-meshheading:6313212-Cloning, Molecular,
pubmed-meshheading:6313212-DNA Restriction Enzymes,
pubmed-meshheading:6313212-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:6313212-Glycoproteins,
pubmed-meshheading:6313212-Nucleic Acid Hybridization,
pubmed-meshheading:6313212-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:6313212-Transcription, Genetic,
pubmed-meshheading:6313212-Trypanosoma brucei brucei,
pubmed-meshheading:6313212-Variant Surface Glycoproteins, Trypanosoma
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pubmed:year |
1983
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pubmed:articleTitle |
Tandem repetition of the 5' mini-exon of variant surface glycoprotein genes: a multiple promoter for VSG gene transcription?
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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