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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1983-11-23
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pubmed:abstractText |
Purine nucleoside phosphorylase (NP; EC 2.4.2.1) deficiency is associated with defective T-cell and normal B-cell immunity. Biochemical mechanisms were investigated by measuring deoxyguanosine and guanosine metabolism in normal T and B lymphoblasts and NP-deficient B lymphoblasts. Deoxyguanosine kinase activity was specifically measured by using an anti-NP antibody to prevent alternative-product formation. Kinase activity towards deoxyguanosine was significantly higher in T-cells, whereas NP activity was similar in both B- and T-cells. Only in T-cells was dGTP produced from exogenous deoxyguanosine, and this was prevented by the simultaneous addition of deoxycytidine, which resulted in a concomitant increase in GTP synthesis. Inhibition by 8-aminoguanosine of NP activity in T lymphoblasts increased formation of dGTP and decreased that of GTP from deoxyguanosine and decreased the formation of GTP from guanosine. These data suggest a central role for deoxyguanosine kinase activity in the T-cell selectivity of the immune defect.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-108044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-108675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-109531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-115901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-14289333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-202960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-208780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-218928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-221924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-311004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-313053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-313056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-382982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-401697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-402573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-408378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-4117384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-48676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-517739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-526339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-53069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-5442271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-5554300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-5781408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6253063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6256370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6256438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6267992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6771283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6772548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6778206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6783354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6795718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6800381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6965300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-6967909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-932021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-96138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312962-99243
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
214
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
711-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6312962-B-Lymphocytes,
pubmed-meshheading:6312962-Cell Line,
pubmed-meshheading:6312962-Chromatography, High Pressure Liquid,
pubmed-meshheading:6312962-Deoxycytidine,
pubmed-meshheading:6312962-Deoxyguanosine,
pubmed-meshheading:6312962-Guanosine,
pubmed-meshheading:6312962-Humans,
pubmed-meshheading:6312962-Pentosyltransferases,
pubmed-meshheading:6312962-Phosphotransferases,
pubmed-meshheading:6312962-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:6312962-Purine Nucleotides,
pubmed-meshheading:6312962-Purine-Nucleoside Phosphorylase,
pubmed-meshheading:6312962-T-Lymphocytes
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pubmed:year |
1983
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pubmed:articleTitle |
The metabolism of deoxyguanosine and guanosine in human B and T lymphoblasts. A role for deoxyguanosine kinase activity in the selective T-cell defect associated with purine nucleoside phosphorylase deficiency.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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