Linked Life Data

6312451

Source:http://linkedlifedata.com/resource/pubmed/id/6312451

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1983-11-23
pubmed:abstractText
Cellular DNA from HLA-typed individuals was digested with the restriction endonucleases HindIII, EcoRV, and EcoRI. The separated restriction endonuclease fragments were hybridized with a HLA class I cDNA probe by using the Southern transfer technique. Digestion of cellular DNA with HindIII generated 22 restriction endonuclease fragments, 11 of which showed polymorphism for presence or absence in a population sample. With EcoRV, 13 fragments were identified; 6 showed polymorphism. EcoRI generated 11 fragments, of which 1 was polymorphic. Of these 18 polymorphic fragments generated by the three restriction endonucleases, each of 5 was found to be positively associated with one allele of the HLA-A or -B allelic series (HLA-Aw24, -B8, -B15, -Bw35, and -B40). One fragment was positively associated with two HLA-A series alleles (HLA-A1 and -A11). Another fragment was positively associated with five HLA-B series alleles (HLA-B5, -B7, -B14, -Bw16, and -Bw35) and one fragment was positively associated with alleles at two loci (HLA-B14 and -Cw5). The serologically defined allele HLA-Aw24 was associated with two polymorphic fragments, one association showing a positive correlation and the other a negative correlation. Each informative family studied thus far has shown segregation of the restriction fragment with the associated serologically defined allele. The fragments associated with serologically defined alleles occurred in the population sample studied at low or moderate frequencies. The remaining polymorphic fragments occur at high frequency, suggesting that class I genes not serologically detected show less polymorphism than serologically defined class I genes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6165999, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6179086, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6219919, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6280871, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6295568, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6297797, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6300865, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6461010, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6571997, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6792704, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6828150, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-6895187, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-7016338, http://linkedlifedata.com/resource/pubmed/commentcorrection/6312451-7063041
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6289-92
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Analysis of HLA class I genes with restriction endonuclease fragments: implications for polymorphism of the human major histocompatibility complex.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't