Linked Life Data

6312352

Source:http://linkedlifedata.com/resource/pubmed/id/6312352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1983-11-23
pubmed:abstractText
With CMP, phosphatidylcholine can be converted to diacylglycerols and CDPcholine by reversal of the cholinephosphotransferase that is normally used for synthesis. Incubation of homogenates of rat brains at pH 8 with 20 mM MgCl2 increased the free fatty acid (FFA) levels 30 to 117%. The FFA levels increased 62 to 212% when 4 mM CMP was included. Diacylglycerols were also produced. Hydrolysis of the diacylglycerols to FFA was markedly inhibited by inclusion of 3 mM diisopropylphosphofluoridate in the incubation mixture. The composition of the fatty acids released by CMP resembles that of phosphatidylcholine except for some polyunsaturated fatty acids. These may have been released from the ethanolamine glycerophospholipids. Most of the CMP-stimulated release of FFA was blocked by inclusion of 1 mM CDPcholine in the incubation mixture. Rat brains were labeled by intracerebral injection of [3H]oleic acid. The labeled oleic acid was released primarily from phosphatidylcholine. Thus, measurements of both mass and radioactivity confirm that the reversal of cholinephosphotransferase followed by diacylglycerol lipase can be an important pathway for the liberation of FFA from phosphatidylcholine.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0364-3190
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
971-81
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
The effect of CMP on the release of free fatty acids of rat brain in vitro.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't