Linked Life Data

6311677

Source:http://linkedlifedata.com/resource/pubmed/id/6311677

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-11-23
pubmed:abstractText
A plasmid, pJL6, was constructed that contains a unique ClaI site twelve codons beyond the bacteriophage lambda cII gene initiation codon. This site allowed us to fuse the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc gene to the amino-terminal portion of the cII gene. Transcription of the hybrid gene is controlled from the phage lambda pL promoter. When this promoter is derepressed, Escherichia coli cells harboring the chimeric plasmid produce a level of cII-myc fusion protein greater than 5% of total cellular protein. Antibodies raised by this protein immunoprecipitate the MC29 gag-myc gene product, P110gag-myc.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
High-level expression in Escherichia coli of the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc protein.
pubmed:publicationType
Journal Article